abstract：:Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

journal_title：Applied and environmental microbiology

authors： Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

更新日期：2019-01-09 00:00:00

abstract：:Hadal environments sustain diverse microorganisms. A few studies have investigated hadal microbial communities consisting of free-living or particle-associated bacteria and archaea. However, animal-associated microbial communities in hadal environments remain largely unexplored, and comparative analyses of animal gut ...

journal_title：Applied and environmental microbiology

authors： Zhang W,Watanabe HK,Ding W,Lan Y,Tian RM,Sun J,Chen C,Cai L,Li Y,Oguri K,Toyofuku T,Kitazato H,Drazen JC,Bartlett D,Qian PY

更新日期：2018-12-13 00:00:00

abstract：:While filamentous cyanobacteria play a crucial role in food web dynamics and biogeochemical cycling of many aquatic ecosystems around the globe, the knowledge regarding the phages infecting them is limited. Here, we describe the complete genome of the virulent cyanophage vB_AphaS-CL131 (here, CL 131), a Siphoviridae p...

journal_title：Applied and environmental microbiology

authors： Šulčius S,Šimoliūnas E,Alzbutas G,Gasiūnas G,Jauniškis V,Kuznecova J,Miettinen S,Nilsson E,Meškys R,Roine E,Paškauskas R,Holmfeldt K

更新日期：2018-12-13 00:00:00

abstract：:Pectin is a complex uronic acid-containing polysaccharide typically found in plant cell walls, though forms of pectin are also found in marine diatoms and seagrasses. Genetic loci that target pectin have recently been identified in two phyla of marine bacteria. These loci appear to encode a pectin saccharification pat...

journal_title：Applied and environmental microbiology

authors： Hobbs JK,Hettle AG,Vickers C,Boraston AB

更新日期：2018-12-13 00:00:00

abstract：:There is growing evidence that anthropogenic sources of antibiotics and antimicrobial-resistant bacteria can spill over into natural ecosystems, raising questions about the role wild animals play in the emergence, maintenance, and dispersal of antibiotic resistance genes. In particular, we lack an understanding of how...

journal_title：Applied and environmental microbiology

authors： Miller EA,Johnson TJ,Omondi G,Atwill ER,Isbell LA,McCowan B,VanderWaal K

更新日期：2018-12-13 00:00:00

abstract：:"Candidatus Methanoperedens nitroreducens" is an archaeon that couples the anaerobic oxidation of methane to nitrate reduction. In natural and man-made ecosystems, this archaeon is often found at oxic-anoxic interfaces where nitrate, the product of aerobic nitrification, cooccurs with methane produced by methanogens. ...

journal_title：Applied and environmental microbiology

authors： Guerrero-Cruz S,Cremers G,van Alen TA,Op den Camp HJM,Jetten MSM,Rasigraf O,Vaksmaa A

更新日期：2018-11-30 00:00:00

abstract：:Freshwater lakes harbor complex microbial communities, but these ecosystems are often dominated by acI Actinobacteria Members of this cosmopolitan lineage are proposed to bolster heterotrophic growth using phototrophy because their genomes encode actino-opsins (actR). This model has been difficult to validate experime...

journal_title：Applied and environmental microbiology

authors： Dwulit-Smith JR,Hamilton JJ,Stevenson DM,He S,Oyserman BO,Moya-Flores F,Garcia SL,Amador-Noguez D,McMahon KD,Forest KT

更新日期：2018-11-30 00:00:00

abstract：:Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...

journal_title：Applied and environmental microbiology

authors： Dupont C,Jumas-Bilak E,Doisy C,Aujoulat F,Chiron R,Marchandin H

更新日期：2018-11-15 00:00:00

abstract：:Receptors on the cell surfaces of bacterial hosts are essential during the infection cycle of bacteriophages. To date, the phage receptors of the industrial relevant dairy starter bacterium Streptococcus thermophilus remain elusive. Thus, we set out to identify cell surface structures that are involved in host recogni...

journal_title：Applied and environmental microbiology

authors： Szymczak P,Filipe SR,Covas G,Vogensen FK,Neves AR,Janzen T

更新日期：2018-11-15 00:00:00

abstract：:Extracellular electron transfer (EET) is a strategy for respiration in which electrons generated from metabolism are moved outside the cell to a terminal electron acceptor, such as iron or manganese oxide. EET has primarily been studied in two model systems, Shewanella oneidensis and Geobacter sulfurreducens Metal red...

journal_title：Applied and environmental microbiology

authors： Conley BE,Intile PJ,Bond DR,Gralnick JA

更新日期：2018-11-15 00:00:00

abstract：:Indirect exposure to waterborne viruses increases the risk of infection, especially among children with frequent hand-to-mouth contacts. Here, we quantified the transfer of one bacteriophage (MS2) and two enteric viruses (adenovirus and coxsackievirus) from liquid to skin. MS2, a commonly used enteric virus surrogate,...

journal_title：Applied and environmental microbiology

authors： Pitol AK,Bischel HN,Boehm AB,Kohn T,Julian TR

更新日期：2018-10-30 00:00:00

abstract：:Pesticides are key stressors of soil microorganisms with reciprocal effects on ecosystem functioning. These effects have been mainly attributed to the parent compounds, while the impact of their transformation products (TPs) has been largely overlooked. We assessed in a meadow soil (soil A) the transformation of iprod...

journal_title：Applied and environmental microbiology

authors： Vasileiadis S,Puglisi E,Papadopoulou ES,Pertile G,Suciu N,Pappolla RA,Tourna M,Karas PA,Papadimitriou F,Kasiotakis A,Ipsilanti N,Ferrarini A,Sułowicz S,Fornasier F,Menkissoglu-Spiroudi U,Nicol GW,Trevisan M,Karpouzas DG

更新日期：2018-10-30 00:00:00

abstract：:Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a...

journal_title：Applied and environmental microbiology

authors： Stamps BW,Nunn HS,Petryshyn VA,Oremland RS,Miller LG,Rosen MR,Bauer KW,Thompson KJ,Tookmanian EM,Waldeck AR,Loyd SJ,Johnson HA,Stevenson BS,Berelson WM,Corsetti FA,Spear JR

更新日期：2018-10-17 00:00:00

abstract：:One hundred seventy-eight mycoplasma strains isolated from South African poultry flocks between 2003 and 2015 were identified by full-genome sequencing and phylogenetic analysis of the 16S rRNA gene and were classified as follows: Mycoplasma gallisepticum (25%), M. gallinarum (25%), M. gallinaceum, (23%), M. pullorum ...

journal_title：Applied and environmental microbiology

authors： Beylefeld A,Wambulawaye P,Bwala DG,Gouws JJ,Lukhele OM,Wandrag DBR,Abolnik C

更新日期：2018-10-17 00:00:00

abstract：:Vector competence refers to the ability of an arthropod to acquire, maintain, and successfully transmit a microbial pathogen. Tick-borne relapsing fever (TBRF) spirochetes are globally distributed pathogens, and most species are transmitted by argasid ticks of the genus Ornithodoros. A defining characteristic in vecto...

journal_title：Applied and environmental microbiology

authors： Krishnavajhala A,Armstrong BA,Lopez JE

更新日期：2018-10-17 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:A mechanistic understanding of how new phenotypes develop to overcome the loss of a gene product provides valuable insight on both the metabolic and regulatory functions of the lost gene. The pgi gene, whose product catalyzes the second step in glycolysis, was deleted in a growth-optimized Escherichia coli K-12 MG1655...

journal_title：Applied and environmental microbiology

authors： McCloskey D,Xu S,Sandberg TE,Brunk E,Hefner Y,Szubin R,Feist AM,Palsson BO

更新日期：2018-09-17 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is critical for genome stability because of its roles in double-strand break repair. Ku and ligase D (LigD) are the crucial proteins in this process, and strains expressing Ku and LigD can cyclize linear DNA in vivo Here, we established a proof-of-concept single-homology-arm linear DNA...

journal_title：Applied and environmental microbiology

authors： Lu S,Nie Y,Wang M,Xu HX,Ma DL,Liang JL,Wu XL

更新日期：2018-09-17 00:00:00

abstract：:Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult to control. Bacillus species have been extensively explored for the biological control of many fungal diseases. In the present study, the marine bacterium Bacillus subtilis BS155 showed a strong an...

journal_title：Applied and environmental microbiology

authors： Zhang L,Sun C

更新日期：2018-08-31 00:00:00

abstract：:Pyocins are bacteriocins secreted by Pseudomonas aeruginosa, and they assist in the colonization of different niches. A major subset of these antibacterial proteins adopt a modular organization characteristic of polymorphic toxins. They include a receptor-binding domain, a segment enabling membrane passage, and a toxi...

journal_title：Applied and environmental microbiology

authors： Ghequire MGK,Öztürk B

更新日期：2018-08-31 00:00:00

abstract：:In this study, the changes in the global proteome of Salmonella in response to desiccation and thermal treatment were investigated by using an iTRAQ multiplex technique. A Salmonella enterica serovar Typhimurium strain was dried, equilibrated at high (1.0) and low (0.11) water activity (aw), and thermally treated at 7...

journal_title：Applied and environmental microbiology

authors： Maserati A,Lourenco A,Diez-Gonzalez F,Fink RC

更新日期：2018-08-31 00:00:00

abstract：:The introduction of "solids" (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a "baby-led" approach to complementary feeding that encourages the early introduction of an adult-type diet results in alteration...

journal_title：Applied and environmental microbiology

authors： Leong C,Haszard JJ,Lawley B,Otal A,Taylor RW,Szymlek-Gay EA,Fleming EA,Daniels L,Fangupo LJ,Tannock GW,Heath AM

更新日期：2018-08-31 00:00:00

abstract：:Escherichia coli, commonly used in chemotaxis studies, is attracted mostly by amino acids, sugars, and peptides. We envisioned modifying the chemotaxis specificity of E. coli by expressing heterologous chemoreceptors from Pseudomonas putida enabling attraction either to toluene or benzoate. The mcpT gene encoding the ...

journal_title：Applied and environmental microbiology

authors： Roggo C,Clerc EE,Hadadi N,Carraro N,Stocker R,van der Meer JR

更新日期：2018-08-31 00:00:00

abstract：:Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combinati...

journal_title：Applied and environmental microbiology

authors： Sakanaka M,Nakakawaji S,Nakajima S,Fukiya S,Abe A,Saburi W,Mori H,Yokota A

更新日期：2018-08-17 00:00:00

abstract：:Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressive...

journal_title：Applied and environmental microbiology

authors： Presley GN,Panisko E,Purvine SO,Schilling JS

更新日期：2018-08-01 00:00:00

abstract：:Azotobacter vinelandii selectively utilizes three types of nitrogenase (molybdenum, vanadium, and iron only) to fix N2, with their expression regulated by the presence or absence of different metal cofactors in its environment. Each alternative nitrogenase isoenzyme is predicted to have different electron flux require...

journal_title：Applied and environmental microbiology

authors： Natzke J,Noar J,Bruno-Bárcena JM

更新日期：2018-08-01 00:00:00

abstract：:Mutacin 1140 belongs to the epidermin family of type AI lantibiotics. This family has a broad spectrum of activity against Gram-positive bacteria. The binding of mutacin 1140 to lipid II leads to the inhibition of cell wall synthesis. Pharmacokinetic experiments with type AI lantibiotics are generally discouraging for...

journal_title：Applied and environmental microbiology

authors： Geng M,Smith L

更新日期：2018-07-17 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is widely used to produce recombinant proteins, taking advantage of this species' high-density cell growth and strong ability to secrete proteins. Circular plasmids containing the P. pastoris-specific autonomously replicating sequence (PARS1) permit transformation of P. pastori...

journal_title：Applied and environmental microbiology

authors： Nakamura Y,Nishi T,Noguchi R,Ito Y,Watanabe T,Nishiyama T,Aikawa S,Hasunuma T,Ishii J,Okubo Y,Kondo A

更新日期：2018-07-17 00:00:00

abstract：:Lysostaphin (Lst) is a potent bacteriolytic enzyme that kills Staphylococcus aureus, a common bacterial pathogen of humans and animals. With high activity against both planktonic cells and biofilms, Lst has the potential to be used in industrial products, such as commercial cleansers, for decontamination. However, Lst...

journal_title：Applied and environmental microbiology

authors： Wu X,Kwon SJ,Kim D,Zha J,Mora-Pale M,Dordick JS

更新日期：2018-07-02 00:00:00

abstract：:Two Baeyer-Villiger monooxygenases (BVMOs), designated BoBVMO and AmBVMO, were discovered from Bradyrhizobium oligotrophicum and Aeromicrobium marinum, respectively. Both monooxygenases displayed novel features for catalyzing the asymmetric sulfoxidation of bulky and pharmaceutically relevant thioethers. Evolutionary ...

journal_title：Applied and environmental microbiology

authors： Zhang Y,Liu F,Xu N,Wu YQ,Zheng YC,Zhao Q,Lin G,Yu HL,Xu JH

更新日期：2018-07-02 00:00:00

abstract：:Cyanobacteria can synthesize alkanes and alkenes, which are considered to be infrastructure-compatible biofuels. In terms of physiological function, cyanobacterial hydrocarbons are thought to be essential for membrane flexibility for cell division, size, and growth. The genetic basis for the biosynthesis of terminal o...

journal_title：Applied and environmental microbiology

authors： Zhu T,Scalvenzi T,Sassoon N,Lu X,Gugger M

更新日期：2018-06-18 00:00:00

abstract：:Although Salmonella enterica can produce life-threatening colitis in horses, certain serotypes are more commonly associated with clinical disease. Our aim was to evaluate the proportional morbidity attributed to different serotypes, as well as the phenotypic and genotypic antimicrobial resistance (AMR) of Salmonella i...

journal_title：Applied and environmental microbiology

authors： Leon IM,Lawhon SD,Norman KN,Threadgill DS,Ohta N,Vinasco J,Scott HM

更新日期：2018-06-18 00:00:00

abstract：:Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

journal_title：Applied and environmental microbiology

authors： Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

更新日期：2018-06-18 00:00:00

abstract：:Human noroviruses are excreted in feces from infected individuals and included in wastewater. It is critical to remove/inactivate them in wastewater treatment processes, particularly in the disinfection step, before release to aquatic environments. However, the high mutation rates of human noroviruses raise concerns a...

journal_title：Applied and environmental microbiology

authors： Rachmadi AT,Kitajima M,Watanabe K,Yaegashi S,Serrana J,Nakamura A,Nakagomi T,Nakagomi O,Katayama K,Okabe S,Sano D

更新日期：2018-06-18 00:00:00

abstract：:The thermoacidophile Acidianus is widely distributed in Yellowstone National Park hot springs that span large gradients in pH (1.60 to 4.84), temperature (42 to 90°C), and mineralogical composition. To characterize the potential role of flexibility in mineral-dependent energy metabolism in contributing to the widespre...

journal_title：Applied and environmental microbiology

authors： Amenabar MJ,Boyd ES

更新日期：2018-05-31 00:00:00

abstract：:The quorum sensing (QS) system controls bacterial group behaviors in response to cell density. In vibrios, LuxR and AphA are two master QS regulators (MQSRs) controlling gene expression in response to high or low cell density. Other regulators involved in the regulation of these two MQSRs and QS pathways remain to be ...

journal_title：Applied and environmental microbiology

authors： Gao X,Wang X,Mao Q,Xu R,Zhou X,Ma Y,Liu Q,Zhang Y,Wang Q

更新日期：2018-05-31 00:00:00

abstract：:Spontaneous vegetable fermentations, with their rich flavors and postulated health benefits, are regaining popularity. However, their microbiology is still poorly understood, therefore raising concerns about food safety. In addition, such spontaneous fermentations form interesting cases of man-made microbial ecosystem...

journal_title：Applied and environmental microbiology

authors： Wuyts S,Van Beeck W,Oerlemans EFM,Wittouck S,Claes IJJ,De Boeck I,Weckx S,Lievens B,De Vuyst L,Lebeer S

更新日期：2018-05-31 00:00:00

abstract：:An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...

journal_title：Applied and environmental microbiology

authors： Watt AE,Browning GF,Legione AR,Bushell RN,Stent A,Cutler RS,Young ND,Marenda MS

更新日期：2018-05-17 00:00:00

abstract：:Ticks are important disease vectors, as they transmit a variety of human and animal pathogens worldwide. Symbionts that coevolved with ticks confer crucial benefits to their host in nutrition metabolism, fecundity, and vector competence. Although over 100 tick species have been identified in China, general information...

journal_title：Applied and environmental microbiology

authors： Wang M,Zhu D,Dai J,Zhong Z,Zhang Y,Wang J

更新日期：2018-05-01 00:00:00

abstract：:A highly virulent Serratia proteamaculans strain, AGR96X, exhibiting specific pathogenicity against larvae of the New Zealand grass grub (Costelytra giveni; Coleoptera: Scarabaeidae) and the New Zealand manuka beetle (Pyronota festiva and P. setosa; Coleoptera: Scarabaeidae), was isolated from a diseased grass grub la...

journal_title：Applied and environmental microbiology

authors： Hurst MRH,Beattie A,Jones SA,Laugraud A,van Koten C,Harper L

更新日期：2018-05-01 00:00:00